品牌 |
Leading Biology |
貨號 |
APR03957G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality POTE Antibody (C-term L446).
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分子量 |
66394 Da
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細胞定位 |
Antigen Cellular Localization:
Cell membrane; Peripheral membrane protein
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宿主 |
Rabbit
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種屬反應性 |
Human
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免疫原 |
438-468 aa
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靶點 |
This POTE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438~468 amino acids from the C-term region of human POTE.
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亞型 |
Rabbit Ig
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通用名 |
A26B3, ANKRD21, POTE
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基因ID |
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UniProt ID |
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總結 |
POTE, is a highly homologous gene family located on numerous chromosomes and expressed in prostate, ovary, testis, placenta, and prostate cancer. The POTE protein contains seven ankyrin repeats between amino acids 140 and 380. Expression of POTE in prostate cancer and its undetectable expression in normal essential tissues make POTE a candidate for the immunotherapy of prostate cancer. The existence of a large number of closely related but rapidly diverging members, their location on multiple chromosomes and their limited expression pattern suggest an important role for the POTE gene family in reproductive processes.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應用 |
WB, IHC-P, E
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稀釋方法 |
WB~~1:500
IHC-P~~1:10~50
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圖像 |
All lanes : Anti-POTE Antibody (C-term L446) at 1:500 dilution
Lane 1: human ovary lysate
Lane 2: human testis lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 66 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with POTE antibody (C-term L446), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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說明書 |
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數(shù)量 |
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