All lanes : Anti-TRIM6 Antibody (N-term) at 1:2000 dilution
Lane 1: 293 whole cell lysate
Lane 2: U-2OS whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 56 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-TRIM6 Antibody (N-term) at 1:2000 dilution
Lane 1: Ramos whole cell lysate
Lane 2: U-2OS whole cell lysate
Lane 3: PC-3 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 56 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
TRIM6 Antibody (N-term) (Cat. APR04586G) western blot analysis in 293,A549,Ramos cell line lysates (35ug/lane).This demonstrates the TRIM6 antibody detected the TRIM6 protein (arrow).