All lanes : Anti-CD300d Antibody at 1:1000 dilution
Lane 1: NCI-H929 whole cell lysate
Lane 2: RPMI 8226 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 22 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.