We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
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This product is a high quality AGR3 Antibody (C-term).
分子量
19171 Da
細胞定位
Antigen Cellular Localization:
Secreted.
宿主
Rabbit
種屬反應(yīng)性
Human
免疫原
119-147 aa
靶點
This AGR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-147 amino acids from the C-terminal region of human AGR3.
AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.
儲存條件
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應(yīng)用
WB, IHC-P, FC, E
稀釋方法
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
圖像
Western blot analysis of AGR3 Antibody (C-term) (Cat. APR06168G) in MCF-7 cell line lysates (35ug/lane). AGR3 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with AGR3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AGR3 Antibody (C-term) (Cat. APR06168G) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.