ETS1 Antibody (N-term)

APR03470G Product Datasheet Protocol

貨號	貨期	規(guī)格 / 價格	詢價
APR03470G		100 μl / ￥4950

大規(guī)格詢價

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品牌	Leading Biology	貨號	APR03470G
產品分類	Polyclonal Antibodies	研究領域
產品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality ETS1 Antibody (N-term).
分子量	50408 Da
細胞定位	Antigen Cellular Localization: Cytoplasm. Nucleus. Note=Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27
宿主	Rabbit
種屬反應性	Human
免疫原	43-70 aa
靶點	This ETS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the N-terminal region of human ETS1.
亞型	Rabbit IgG
通用名	EWSR2
基因ID	2113
UniProt ID	P14921
功能	Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.
總結	ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (summary by Dwyer et al., 2007 [PubMed 17986575]).
儲存條件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
應用	WB, IF, FC, E
稀釋方法	WB~~1:1000 FC~~1:10~50 IF~~1:10~50
圖像	All lanes : Anti-ETS1 Antibody (N-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysates Lane 2: 293T whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST. ETS1 Antibody (N-term) (Cat. APR03470G) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Confocal immunofluorescent analysis of ETS1 Antibody (N-term)( APR03470G) with MCF-7 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
說明書	Product Datasheet Protocol
數(shù)量

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