APR04867G staining HNRNPUL2 in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-HNRNPUL2 Antibody (Center) at 1:2000 dilution
Lane 1: MCF-7 whole cell lysates
Lane 2: Jurkat whole cell lysates
Lane 3: 293T/17 whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 85 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-HNRNPUL2 Antibody (Center)at 1:8000 dilution + Hela whole cell lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 85 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.