品牌 |
Leading Biology |
貨號(hào) |
AMM08112G |
產(chǎn)品分類 |
Monoclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality TBB1 antibody.
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分子量 |
50327 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm, cytoskeleton
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宿主 |
Mouse
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種屬反應(yīng)性 |
Human, Mouse
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靶點(diǎn) |
This monoclonal antibody is generated from mice immunized with a recombinant protein human TBB1.
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克隆號(hào) |
86CT4.4.4
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亞型 |
Mouse IgG1
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基因ID |
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UniProt ID |
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功能 |
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
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總結(jié) |
This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.
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形式 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, IHC-P, IF, E
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稀釋方法 |
WB~~1:100~500
IHC-P~~1:10~50
IF~~1:10~50
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別名 |
Tubulin beta-1 chain, TUBB1
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圖像 |
All lanes : Anti-TBB1 Antibody at 1:1000 dilution
Lane 1: CCRF-CEM whole cell lysate
Lane 2: Hela whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of anti-TBB1 Antibody (Cat. AMM08112G) in mouse brain tissue, Y79, CEM and 293 lysates (35μg/lane). TBB1 (arrow) was detected using the purified Mab.(1μg/ml)
Formalin-fixed and paraffin-embedded human brain reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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說明書 |
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數(shù)量 |
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