品牌 |
Leading Biology |
貨號 |
AMM08356G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality TSPAN2 antibody (Center).
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分子量 |
24148 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Membrane; Multi-pass membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
109-137 aa
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靶點(diǎn) |
This TSPAN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-137 amino acids from the Central region of human TSPAN2.
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亞型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
May play a role in signalling in oligodendrocytes in the early stages of their terminal differentiation into myelin-forming glia and may also function in stabilizing the mature sheath.
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總結(jié) |
TSPAN2 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, FC, E
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稀釋方法 |
WB~~1:1000
FC~~1:10~50
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別名 |
Tetraspanin-2, Tspan-2, Tetraspan NET-3, TSPAN2
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圖像 |
Anti-TSPAN2 Antibody (Center) at 1:1000 dilution + K562 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 24 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
TSPAN2 Antibody (Center) (Cat. AMM08356G) western blot analysis in CEM,K562,HL-60 cell line lysates (35ug/lane).This demonstrates the TSPAN2 antibody detected the TSPAN2 protein (arrow).
TSPAN2 Antibody (Center) (Cat. AMM08356G) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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說明書 |
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數(shù)量 |
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