品牌 |
Leading Biology |
貨號 |
APR11425G |
產(chǎn)品分類 |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality AOC3 antibody (Center).
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分子量 |
84622 Da
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細(xì)胞定位 |
Antigen Cellular Localization:
Cell membrane; Single-pass type II membrane protein
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
613-640 aa
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靶點 |
This AOC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613-640 amino acids from the Central region of human AOC3.
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亞型 |
Rabbit Ig
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通用名 |
VAP1
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基因ID |
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UniProt ID |
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功能 |
Cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has semicarbazide-sensitive (SSAO) monoamine oxidase activity. May play a role in adipogenesis.
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總結(jié) |
Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity.
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形式 |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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儲存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
IHC-P, WB, FC, E
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稀釋方法 |
IHC-P~~1:10~50
WB~~1:1000
FC~~1:10~50
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別名 |
Membrane primary amine oxidase, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase, SSAO, Vascular adhesion protein 1, VAP-1, AOC3, VAP1
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圖像 |
APR11425G staining AOC3 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-AOC3 Antibody (Center) at 1:2000 dilution
Lane 1: human lung lysate
Lane 2: human liver lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 85 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of AOC3 Antibody (Center) (Cat. APR11425G) in CEM cell line lysates (35ug/lane).AOC3 (arrow) was detected using the purified Pab.
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說明書 |
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數(shù)量 |
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