品牌 |
Leading Biology |
貨號(hào) |
APR08180G |
產(chǎn)品分類(lèi) |
Polyclonal Antibodies |
研究領(lǐng)域 |
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產(chǎn)品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality LDHA antibody (Center).
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分子量 |
H=40,30,27,37;R=36 KDa
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細(xì)胞定位 |
Antigen Cellular Localization:
Cytoplasm.
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宿主 |
Rabbit
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種屬反應(yīng)性 |
Human
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免疫原 |
154-182 aa
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靶點(diǎn) |
This LDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-182 amino acids from the Central region of human LDHA.
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基因ID |
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UniProt ID |
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總結(jié) |
LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria.
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形式 |
Liquid. In PBS containing 0.02% sodium azide.
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儲(chǔ)存條件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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應(yīng)用 |
WB, FC, IHC-P
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稀釋方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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別名 |
L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA
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圖像 |
All lanes : Anti-LDHA Antibody (Center) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: A375 whole cell lysate
Lane 3: human placenta lysate
Lane 4: COS-7 whole cell lysate
Lane 5: A431 whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 36 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human skin tissue reacted with LDHA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
LDHA Antibody (Center) (Cat. APR08180G) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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說(shuō)明書(shū) |
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數(shù)量 |
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